Part:BBa_K1316006:Design

Rhamnose promoter coupled to N-genes

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The Rhamnose promoter was used to inducibly regulate the expression of the N-genes A strong RBS was placed in front of each gene An "all frame STOP" region was placed after each gene nfsA and nfsB have a 6xHis tag at the N-terminus; nemA has a 6xHis tag at the C-terminus. This way, the proteins can be purified to check if they are successfully expressed

Source

"De novo" synthesis